Spontaneous Activities From Cultured Cerebellum
A: Cerebellar slice cultured on a MED probe (left). The slice was prepared from 10-day old rat and cultured for 10 days on a membrane, then transferred to the MED probe. The diagram (right) shows the microanatomy with respect to the electrode arrangement.
B: Spontaneous activity recorded from 2 different channels. The recording sites and corresponding channels within the slice are indicated in the schematic diagram and image above. Single units can be studied for extended periods of time (up to 2 weeks). The synaptic physiology and pharmacology of cerebellar neurons can be evaluated over several weeks. Cultures on MED64 probes can be viable for several months.