Evaluation of anti-epilepsy drug effects on iPSC-derived neurons
Induction and suppression of epileptiform activities are examined using the MED64.
A: Pentylenterazole (PTZ) and then phenytoin (an anti-epilepsy drug) is administrated to iPSC-derived neuron cultured on the MED Probe (20 weeks in culture) with cumulative concentrations. Action potentials are recorded from all 64 electrodes, and the distribution of recorded action potentials across 64 electrodes are displayed in raster plots (appearance of black vertical lines indicates synchronized bursts (SB)).
B: Summary for the change of spike rate (with red bar for PTZ while blue bar for phenytoin) and number of bursts for 5 minutes. PTZ induce rapid increase both for spikes and SB, that is suppressed with phenytoin.