Long-term recording of LTP in cultured hippocampal slices.
Ken Shimono a , Michel Baudry a, b , Lam Ho a , Makoto Taketani a , Gary Lynch b
Neural Plasticity. 2002;9(4):249-54. (late publication)
a Tensor Biosciences, 101 Theory Dr., Suite 250, Irvine, CA 92612, USA
b Department of Psychiatry and Human Behavior, University of California, Irvine, CA 92697, USA
Long-term potentiation (LTP) was elicited by high frequency stimulation in hippocampal slices cultured on multi-electrode arrays. LTP lasting more than 1 h was recorded in 75% of slices, and a significant number of slices exhibited a non-decaying LTP that lasted more than 48 h. LTP induction was completely and reversibly blocked by an antagonist of the NMDA receptor, APV. Our results suggest the possibility of using chronic recording in hippocampal slices cultured on multi-electrode arrays to study the mechanisms underlying LTP maintenance and stabilization.
