Chronic multichannel recordings from organotypic hippocampal slice
cultures: protection from excitotoxic effects of NMDA by noncompetitive
NMDA antagonists
Ken Shimono a,*, Michel Baudry b, Victor Panchenko a, Makoto Taketani a
Journal of Neuroscience Methods 120 (2002) 193-202
a Tensor Biosciences, 101 Theory Dr., Suite 250, Irvine, CA 92612, USA
b Department of Psychiatry and Human Behavior, University of California, Irvine, CA 92697, USA
In vitro neuronal damage has traditionally been evaluated by biochemical or anatomical but not by electrophysiological techniques. In the present study, we combined two newly developed technologies, an 8x8 multi-electrode array (MED-64) and cultured hippocampal slices, to demonstrate the potential use of electrophysiological measures as index of neuronal damage. We first demonstrated the stability of electrophysiological recordings over prolonged periods of time (up to 14 days) in field CA1 of cultured hippocampal slices following electrical stimulation of the Schaffer collateral pathway. We then assessed the neurotoxic properties of NMDA and AMPA and determined that the time-course, potency, and efficacy of these two neurotoxins were similar to those assessed by other experimental approaches. We also compared the efficacy and potency of two non-competitive NMDA receptor antagonists to protect against NMDA-mediated neurotoxicity. Again, the results matched well with the results obtained from traditional techniques. Thus, this new technology might provide a new and powerful method to study the chronic effects of drugs or other experimental manipulations in an in vitro preparation. © 2002 Elsevier Science B.V. All rights reserved.
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