Modulation of Field Potentials Evoked in Mouse Brain Striatatal Tissue Slices Using Planar Microelectrode Array
G.D. Ritchie*; S.M. McInturf; F.J. McDougle; E.M. Krouse; M.Y.-V. Bekkedal; J. Rossi III
Society for Neuroscience, 344.17 (2002)
Neurobehavioral Effects Laboratory, Naval Health Research Center, Wright-Patterson AFB, OH, USA
A Panasonic MED-64 multi-electrode array system was used to evaluate the possibility that evoked field potentials could be recorded from striatal brain slices. Tissue slices transecting the striatum were taken from the brains of Swiss-Webster mice. The slices were aligned on 64 electrode planar microelectrode arrays such that either a discrete nucleus (caudate, globus pallidus or nucleus accumbens) or an inter-area projection (nucleus accumbens to globus pallidus or amygdala to nucleus accumbens) was registered with the highest possible electrode spacing density (150, 300 or 450 uM spacings). Individual electrodes for stimulation and recording were determined for each slice by assessing field potential amplitudes after 80 uA stimulations of the best anatomically positioned electrodes, and choosing the electrode pair that produced the most robust potentials. Following determination of the electrode pair, baseline amplitudes were assessed through stimulation once every 10 sec for 5 min. Following baseline acquisition, the slices were statically treated with trimethylolpropane phosphate (TMPP) at concentrations ranging from 10-30 uMol, and were further evaluated for 5 additional minutes. Sixty min following flushing with untreated media, the slices were tested identically to the baseline procedure. TMPP was found to reliably increase the amplitude of the evoked potentials in a dose response fashion. Following the washout procedure, the amplitude of the potentials were found to be slightly attenuated from max TMPP amplitudes.
Supported by: Defense Advanced Research Projects Agency
