 |
||||
 |
 |
 |
||
 |
A low-noise multi-electrode array system for in vitro extracellular electrophysiology |
|||
 |
||||
 |
||||
EXPERIMENTAL METHODS
Treatment
Polyethylenimine (PEI) Methods
1. Treat the surface of the MED probe with 0.1% PEI in 25 mM borate buffer, pH 8.4, for overnight at room temperature.
2. Rinse three times with sterile distilled water.
0.1% polyethylenimine solution in 25 mM borate buffer (pH8.4)
1) Borate Buffer
- Dissolve sodium borate (Na2B4O7/10H2O; 9.525g for 1L) in DDW
- Adjust pH to 8.4 with 1N HCl
- Keep in refrigerator (4C)
2) 0.1% polyethylenimine (PEI) solution
- Dilute polyethylenimine (SIGMA, P-3143 50% aqueous solution) to 0.1% with borate buffer
1. Oka, H. et al., J. Neursci. Meth. 93, 61-67, 1999
2. Shimono, K. et al., J. Neurosci. Meth.120: 192-202, 2002
 |
HOME PRODUCTS APPLICATIONS PUBLICATIONS SUPPORT CONTACT ABOUT US |
|