Treatment
Polyethylenimine (PEI) Methods
1. Treat the surface of the MED probe with 0.1% PEI in 25 mM borate buffer, pH 8.4, for overnight at room temperature.
2. Rinse three times with sterile distilled water.
3. Treat with buffering solution (e.g. PBS, culture medium, etc), containing 10 % fetal bovine serum and 10 % horse serum, for at least 1 hr at 37 C
(0.1% polyethylenimine solution in 25 mM borate buffer)
1) Borate Buffer
- Dissolve sodium borate (Na2B4O7/10H2O; 9.525g for 1L)
- Adjust pH to 8.4 with 1N HCl
- Keep in refrigerator (4C)
2) 0.1% polyethylenimine (PEI) solution
- Dilute polyethylenimine (SIGMA, P-3143 50% aqueous solution) to 0.1% with borate buffer
1. Oka, H. et al., J. Neursci. Meth. 93, 61-67, 1999
2. Shimono, K. et al., J. Neurosci. Meth.120: 192-202, 2002
