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A low-noise multi-electrode array system for in vitro extracellular electrophysiology |
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Experiment-based Applications
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Preparation-Based Applications
MED64 APPLICATIONS
Recording Single Unit Activity
Example 1: Recording of single unit activity from substantia nigra
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A: Micrograph of a horizontal midbrain slice containing the substantia nigra, placed over the 8 x 8 array of electrodes on a MED probe. B: Spontaneous single unit activity acquired at all 64 channels. C: Spontaneous activity acquired at channel 44. The expanded traces show spikes corresponding to two individual cells (A and B) recorded at the same channel. Courtesy of Dr. Nicola Beretta, Fondazione Santa Lucia IRCCS, Rome, Italy |
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Example 2: Recording from neuron cultures
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A: Spontaneous single-unit activities obtained from dissociated hippocampal neurons cultured for 30 days in a MED probe. B: Spike sorting for a single channel (9) performed by Mobius software. Spikes were extracted based on a +/-20µV threshold and clustered based on waveform similarity. 4 different spikes are detected in this channel and their individual frequencies are displayed on the bottom. |
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Example 3 - Dissociated cell culture of rat suprachiasmatic nucleus
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Dissociated cells from the suprachiasmatic nucleus of newborn rat pups were cultured on the MED probe for 2-6 weeks (left).
Spontaneous neural activity began to be monitored 4-10 days after cell culturing and was continuously recorded for 2-6 weeks thereafter. Spontaneous firing frequencies with periodic circadian oscillations were frequently observed (center).
In the study, using data of at least 5 consecutive days of recording for every cell, most neurons (67/88) showed robust circadian rhythmicity, as assessed by chi-square periodogram analysis (right).
Based on Honma, S. et. al. (1998) Neuroscience Letters , 250 ,157-160.
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