Left: A phase-contrast photomicrograph of
a cultured SCN slice on the MED probe after 14 d in culture.
(MED-P210A, 100 mm inter-polar
distance) Scale bar, 150 m
m
Right:
Representative circadian firing rhythms of SCN neurons from
Clock mutant and wild-type mice in slice
cultures.
The
firijng rhythm was expressed in a histogram of the mean firing
rate in 15 min. and double-plotted. The numbers in the right
margin of the first lane in each panel were the scales
of firing rate. The circadian periods of these firing rhythms
were 28.1 h (left) and 28.0 h (right) for Clock/Clock, 25.3 h
and 24.5 h for Clock/+, and 23.3 h and 23.8 h for +/+ neurons,
respectively.
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