Experiment-based Applications

Synaptic Plasticity

Single Unit Activity

Rhythmic Activity

Muscle Propagation
and Pacing

QT Prolongation

Pharmacology and
Drug Testing

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Preparation-Based Applications

PREPARATION BASED APPLICATIONS

Slice Cultures –Hippocampus–

Publications for this application

Example 1: Chronic drug effects on cultured rat hippocampal slices

A	B
C

A: Rat Hippocampal slice cultured directly on a MED probe (MED-P530A; 300μm inter-polar distance, 10 days in culture)

B: Extracellular field potentials in CA1 evoked by paired-pulse stimulation. Responses remained stable over days.

C: Comparison of the effects of various chronic treatments on synaptic responses. fEPSPs were measured in cultured hippocampal slices 3 days after different drug treatments. fEPSP amplitude was expressed as a percent of the pre-drug baseline response for each slice. Co-incubation with NMDA antagonists, such as 1μM MK801and 30μM Memantine, blocked the excitotoxic action of NMDA (10μM).

Shimono K et al. J Neurosci. Methods 2002, 120(2): 193-202

Example 2: Longevity of LTP in cultured hippocampal slices

A. Hippocampal slices cultured on the MED probe (150µm inter-polar distance)

B. Field EPSPs evoked by Schaffer/Commissural stimulation were recorded in field CA1 before and after tetanic stimulation.

C. Summary graph showing the stability of LTP over several days. The amplitude of the fEPSP (calculated as a percentage of averaged baseline values; means ±S.E.M., n=8) , is shown for the control and potentiated pathways. Arrows indicate the time of tetanus stimulation.

D. LTP amplitude was averaged over a 10 min period at 1h, 24h, and 48h after tetanus stimulation, and was expressed as percentage of baseline values.

Shimono K et al., Neural Plasticity 2002, 9(4) 249-254