MED64 multi-electrode array system
A complete, user-friendly solution for in-vitro electrophysiology
based on planar microelectrode technology.
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Preparation Based Applications

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Slice cultures     -Hippocampus-                                                Publication list     

 

Example 1: Evaluation of Chronic Drug Effects on Cultured Rat Hippocampal Slices 

  

Left: Rat hippocampal slice cultured directly on MED probe  (300 mm inter-polar distance, 10 days in culture) 
Right: Stable response from cultured slice for three days. Paired-pulse stimulations with same intensity were delivered to one electrode at various period of time.
 


Results of chronic drug application to the slice.
Decrease in amplitude of response in the presence of 10 mM NMDA. 
Co-incubation with NMDA antagonists, such as 1 mM MK801and 30 mM Memantine, blocked the excitotoxic action  of NMDA.

                                                                                    Shimono K et al.,J Neurosci. Methods 2002, 120(2): 193-202

Download PDF (461 kB) of above application. 

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Example 2: Longevity of LTP in Cultured Hippocampal Slices

A.Hippocampal slices cultured on the MED probe (150 µm inter-electrode spacing)

B. Field EPSPs evoked by Schaffer/Commisural stimulation were recorded in field CA1 before and after tetanic stimulation.

C.Summary graph showing the stability of LTP over several days. The amplitude of the fEPSP (calculated as a percentage of averaged baseline values; means ±S.E.M., n=8) , is shown for the control and potentiated pathways. Arrows indicate the time of tetanus stimulation.

D.LTP amplitude was averaged over a 10 min period at 1h, 24h, and 48h after tetanus stimulation, and was expressed as percentage of baseline values.

Shimono K et al., Neuroal Plasticity 2002, 9(4) 249-254

Link to publication list of Hippocampus slice clutures.

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