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Example 1: Evaluation of Chronic
Drug Effects on Cultured Rat Hippocampal Slices
Left:
Rat hippocampal slice cultured directly on MED
probe
(300 mm
inter-polar distance, 10 days in culture）
Right: Stable response from cultured slice
for three days. Paired-pulse
stimulations with same intensity were delivered to one
electrode at various period of time.
Results
of chronic drug application to the slice.
Decrease
in amplitude of response in the presence of 10
mM
NMDA.
Co-incubation
with NMDA antagonists, such as 1 mM
MK801and 30 mM
Memantine, blocked the excitotoxic action of
NMDA.
Shimono
K et al.,J
Neurosci. Methods
2002, 120(2): 193-202
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Example 2: Longevity of LTP in Cultured Hippocampal
Slices
A.Hippocampal slices cultured on the MED probe (150 µm
inter-electrode spacing)
B. Field EPSPs evoked by
Schaffer/Commisural stimulation were recorded in field CA1
before and after tetanic stimulation.
C.Summary graph
showing the stability of LTP over several days. The amplitude
of the fEPSP (calculated as a percentage of averaged baseline
values; means ±S.E.M., n=8) , is shown for the control and
potentiated pathways. Arrows indicate the time of tetanus
stimulation.
D.LTP amplitude was averaged over a 10 min
period at 1h, 24h, and 48h after tetanus stimulation, and was
expressed as percentage of baseline values.
Shimono
K et al., Neuroal Plasticity 2002, 9(4)
249-254
Link to publication list of Hippocampus slice
clutures.
