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A low-noise multi-electrode array system for in vitro extracellular electrophysiology |
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Experiment-based Applications
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Preparation-Based Applications
MED64 APPLICATIONS
QT Prolongation
Example : Evaluation of QT prolongation with iPS/ES cell-derived cardiomyocytes
- QT prolongation can be evaluated by measuring field potential prolongation with the MED64 system.
- Cardiac myocytes differentiated from iPS/ES cells can be placed or cultured directly on a MED probe.
- Sophisticated on-line analysis with Mobius QT software.
Analysis of Field potential signals recorded with the MED64
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The filed potential signals acquired with the MED64 can consists of four components of 2-4 explained on the left figure and, can vary depending on the balance of each component. However, the typical waveform shows depolarization phase followed by repolarization phase as seen in the 1 on the left figure. The time from the depolarization to repolarization (Filed potential duration) indicate QT interval and QT prolongation can be evaluated by measuring the filed potential prolongation. Filed Potential Prolongation
Field potential prolongation obtained from chick embryo cardio myocyte in the absence (blue) and presence (pink) of 200 nM E4031, a well-studied hERG channel blocker. In the presence of 200 nM E4031, the action potential duration was prolonged, and the slope of the rising and descending phase are decreased. |
Evaluation of drug-induced field potential prolongation with iPSC-derived cardiomyocytes
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[Top] Human iPSC-derived cardiomyocytes on a MED probe (MED-P515A: 150 μm inter-polar distance) [Middle] The left graph shows signals acquired from electrode 29 in the absence (Blue) and presence of E4031 (Purple: 100 nM, Green: 1 μM). E4031 prolongs the waveform and decreases rising and descending slopes in a dose-dependent manner. The time between peaks for depolarization and repolarization phases (“field potential duration”; black bar) of the waveform is automatically measured and graphed by Mobius software (right panel). Yellow bars represent user-defined experimental phases with different E4031 concentrations used for computing the averaged values below. [Bottom] Average and SD of field potential duration computed automatically by Mobius software for above data. |
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