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A low-noise multi-electrode array system for in vitro extracellular electrophysiology |
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Experiment-based Applications
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Preparation-Based Applications
MED64 APPLICATIONS
Pharmacology and Drug Safety Screening
Example 1: Measuring ’regional‘ drug effects from different CNS synapses
A. Schematic diagram showing multiple stimulation and recording sites along the hippocampal tri-synaptic loop.
B. The effects of an AMPA modulator (250µM) on test and paired-pulse responses (mono-synaptic fEPSPs) from different circuits in the rat hippocampus. The representative traces in black are taken before, and those in purple are taken after application of the drug.
Example 2: Drug effects on SCN neurons
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[Left] Micrograph of a rat SCN slice on MED-P515A probe (150μm inter-polar distance). [Right] Spontaneous activity of SCN neurons recorded at the electrode marked in red. The activity decreased in the presence of serotonin. [Bottom] Serotonin reduces the rate of spontaneous firing in a dose-dependent manner. The rate of spontaneous activity seen in the baseline period (approximately 4 Hz) decreased noticeably as the serotonin concentration was increased from 1 to 10µM. Partial recovery was observed after washing out the drug. |
Example 3: Chronic drug effects on cultured rat hippocampal slices
A: Rat Hippocampal slice cultured directly on a MED probe (MED-P530A; 300μm inter-polar distance, 10 days in culture)
B: Extracellular field potentials in CA1 evoked by paired-pulse stimulation. Responses remained stable over days.
C: Comparison of the effects of various chronic treatments on synaptic responses. fEPSPs were measured in cultured hippocampal slices 3 days after different drug treatments. fEPSP amplitude was expressed as a percent of the pre-drug baseline response for each slice. Co-incubation with NMDA antagonists, such as 1μM MK801and 30μM Memantine, blocked the excitotoxic action of NMDA (10μM).
Example 4: Drug effects on co-cultured slices of septum and hippocampus
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A. Septal slices (bottom part, from 5 day-old rats) were co-cultured with hippocampal slices. (upper part, from 9 day-old rats) on the MED probe for 19 days (450µm inter-electrode spacing).
B. Spontaneous unit activity measured at the electrodes marked in red. Physostigmine 1µM, a cholinesterase inhibitor, increased activity in both CA1 and CA3. Atropine 1µM, a muscarinic receptor antagonist, blocked activity.
C. Quantification of the effects of Physostigmine and atropine. (means + SEM n=2). This result indicates that spontaneous activity in septo-hippocampal co-cultures is influenced by cholinergic drugs.
Example 5: Effect of capsaicin on DRG neuron firing
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A. Rat dorsal root ganglion (DRG) neurons cultured directly on MED probe. (150μm inter-polar distance; 3 days in culture)
B. Spontaneous unit activity obtained from the cultured DRG neurons. Activity is elicited briefly for a few seconds by 1 µM Capsaicin.
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