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Example 1: Long Term Potentiation in Mouse
Hippocampal Slices
*Simply place the slice
on the MED probe and you are ready to record! (No need to
manipulate grass electrodees).
*No need for a special
shielded environment. Conduct your experiment everyday under
stable low-noise levels.
*Look for the best stimulation point
easily by using the control panel in front of the
amplifier.
*Conduct your experimetns and get on-line
analysis using MED64 Mobius software.
Top left: Micrograph of a
hippocampal slice obtained from a C57BL6 mouse placed on MED-P515A probe
(150 m m
inter-polar distance) and centered on apical region of field CA1. Population EPSPs (pEPSPs) are elicited by a single pulse stimulation to the electrode marked in blue and responses are recorded at the other 63 electrodes.
Bottom: The time course of the slope measured at the electrode marked in red taken from before (green) and after (red) a theta burst stimuation.
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Example 2: Measuring
’Regional‘ Drug Effects from Different CNS Synapses
Left. Schematic showing multiple stimulating and
recording sites along the hippocampal tri-synaptic loop.
Right.
The effects of an AMPA modulator (250 µM) on test and
paired-pulse responses (mono-synaptic fEPSPs) from different
circuits in the rat hippocampus. The representative traces in
black are taken before, and those in purple are taken after
application of the drug.
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Example
3: Spatial Recording of Drug Effect on Cholinergic
Rhythms
Top left: Micrograph of hippocampal slice on MED-P545A
probe (450 mm inter-polar
distance)
Top right: A
shows b rhythm activities at 20
electrodres (blue squqre in the top-left picture) induced by 50
mM carbachol,
while B shows the activities in the same area after adding 3
mM diazepam. Rhythmic activities
were greatly enhanced by diazepam and spread to regions that
were relatively inactive.
Bottom: The superimposed
power spectra, sampled in the absence (blue) and presence
(red) of diazepam, indicate that diazepam induces a much
greater increase in amplitude of the power spectra in CA1 than
in CA3.
(x-axis: Frequency, y-axis:
Amplitude)
K. Shimono et al. Brain Res., 2002,
950(1-2):279-287
